Composite

Part:BBa_K4889006:Design

Designed by: zhaoxia zhang   Group: iGEM23_Shanghai-MedX   (2023-09-07)


Plac-GST-MST2 (mutant)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1685
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 939
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 765
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1616
    Illegal SapI.rc site found at 169


Design Notes

The part includes T7 promoter, lac operator, RBS, GST tag, MST2 (1-308) (mutant), and T7 terminator. MST2 (1-308) (mutant) was digested with restriction endonucleases (NcoI and XhoI) and ligated into the plasmid containing the other components using T4 DNA ligase.


Source

The part is Composite pasts.

References